Flow sorting and culture of plant protoplasts
作者:
Kristi R. Harkins,
David W. Galbraith,
期刊:
Physiologia Plantarum
(WILEY Available online 1984)
卷期:
Volume 60,
issue 1
页码: 43-52
ISSN:0031-9317
年代: 1984
DOI:10.1111/j.1399-3054.1984.tb04247.x
出版商: Blackwell Publishing Ltd
关键词: Flow cytometry;fluorescence;Nicotiana;regeneration;tissue culture
数据来源: WILEY
摘要:
Conditions have been established for the rapid flow analysis of leaf protoplasts ofNicotiana tabacumL. cv. Xanthi using a flow cytometer‐cell sorter. A procedure based upon chlorophyll autofluorescence was devised to permit the systematic evaluation of flow conditions in order to identify those under which protoplast damage was minimized. These conditions were employed for the flow sorting of protoplasts, following which it was possible to regenerate the sorted protoplasts into complete plants. The application of flow sorting is discussed for the rapid identification and selection of somatic hybrids produced by protoplast fusio
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