DNA amplification from vegetative and sexual tissues of trees using polymerase chain reaction
作者:
Jean Bousquet,
Luc Simon,
Maurice Lalonde,
期刊:
Canadian Journal of Forest Research
(NRC Available online 1990)
卷期:
Volume 20,
issue 2
页码: 254-257
ISSN:0045-5067
年代: 1990
DOI:10.1139/x90-037
出版商: NRC Research Press
数据来源: NRC
摘要:
A simple protocol for the extraction of total DNA from minute amounts of tissues and subsequent amplification of specific sequences by polymerase chain reaction is presented. The method is applicable to a wide variety of vegetative tissues such as leaves, single needles and rootlets, cell suspensions, and also single sexual embryos and megagametophytes derived from a variety of gymnosperms and perennial angiosperms. Amplification of DNA is shown using pairs of primers specific to genes that encode the small ribosomal subunit.
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