Enhanced Activator Activity of the Mixture of Streptokinase and a Modified Form of Plasminogen (Lys-Plasminogen) in the Presence of Fibrin: Role of Conformational Change of Plasminogen
作者:
Akikazu Takada,
Yumiko Takada,
Yoshiaki Sugawara,
期刊:
Pathophysiology of Haemostasis and Thrombosis
(Karger Available online 1988)
卷期:
Volume 18,
issue 2
页码: 106-112
ISSN:1424-8832
年代: 1988
DOI:10.1159/000215790
出版商: S. Karger AG
关键词: Lys-plasminogen;Streptokinase;Fibrin;Fibrinogen;Fibrinogen degradation product
数据来源: Karger
摘要:
Equimolar concentrations of Lys-plasminogen (Lys-PLG) and streptokinase (SK) were mixed with various concentrations of fibrin, fibrinogen, fragment D or E (potentiating agents). The activity of the mixture was measured by the hydrolysis of S-2251. Kinetic analyses indicated that catalytic rate constant (kcat) of the hydrolysis of S-2251 increased in the presence of increasing amounts of potentiating agents. Km D. It could be concluded that a complex of SK, Lys-PLG and potentiating agent is a better enzyme, thus activator, than a complex of SK and Lys-PLG. Since Lys-PLG does not change its conformation and its activation rate upon interaction with fibrin, the enhanced activator activity of complex of SK, Lys-PLG and potentiating agent may not be due to the conformational change of Lys-PLG.
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