Colorimetric Evaluation of Cultured Osteoblast-like Cells (ROS 17/2.8)
作者:
GentyChristian,
PalleSabine,
VanelleLaurence,
BourrinSandrine,
AlexandreChristian,
期刊:
Biotechnic&Histochemistry
(Taylor Available online 1994)
卷期:
Volume 69,
issue 3
页码: 160-164
ISSN:1052-0295
年代: 1994
DOI:10.3109/10520299409106279
出版商: Taylor&Francis
关键词: osteoblast-like-cells. methylene blue;cell quantification;spectrophotometry;scanning microscopy
数据来源: Taylor
摘要:
We have developed a colorimetric method for evaluating the number of osteoblastic cells in culture without destroying the cells. This assay is based on the staining of basophilic cellular compounds with methylene blue. The dye bound by the cells is released at low pH and measured in a spectrophotometer at 662 nm. Linear correlations exist between the absorbance measured by the methylene blue assay and the number of cells seeded, the total cellular protein content, and thymidine labeling. This colorimetric method has the advantage of preserving cell integrity. After destaining, scanning electron microscopy can be performed on well preserved cell morphology.
点击下载:
PDF (1395KB)
返 回