Capillary zone electrophoresis with amperometric detection was employed for the determination of lincomycin and lincomycin B in bulk drug and pharmaceutical formulations. The electrode used was a 300μm diameter Cu disk electrode operated in a wall-jet configuration. With an applied potential of +675mV (vs Ag/AgCl) and 80cm capillary(i.d. 25μm) at 26kV in the electrophoretic medium of 0.lOmol/L NaOH, lincomycin and lincomycin B could be completely separated within 15 minutes with resolution of 2.3. The response was linear over the concentration of 10.0–150.0μg/mL with a correlation coefficient of 0.9998, and the detection limit of 1.0μg/mL for lincomycin. The results of analysis were 98.2–99.3% of lincomycin label claims, and compared favourably with those obtained by the official method. The method is simple, rapid and precise for routine analysis of underivatized lincomycin and lincomycim B in bulk drug and pharmaceutical formulations.