An Update on Protein Stains: Amido Black, Coomassie Blue G, and Coomassie Blue R
作者:
WilsonCurtis M.,
期刊:
Biotechnic&Histochemistry
(Taylor Available online 1992)
卷期:
Volume 67,
issue 4
页码: 224-234
ISSN:1052-0295
年代: 1992
DOI:10.3109/10520299209110070
出版商: Taylor&Francis
关键词: protein stains;amido black;Coomassie blue G;Coomassie blue R;dye impurities;gel electrophoresis;protein assay;dye binding
数据来源: Taylor
摘要:
Samples of amido black, Coomassie blue G, and Coomassie blue R obtained over a number of years were tested for dye content, impurities, and effectiveness for staining proteins after polyacrylamide gel electrophoresis and for protein dye-binding assays. Some impurities produced reactions resembling metachromasia with specific proteins, although instances of true metachromatic staining are also reported. Several simple assays are given for determining dye content and relative levels of impurities. Recommendations are made for selecting batches of commercial dyes which are most likely to perform satisfactorily.
点击下载:
PDF (1577KB)
返 回