Activation of Noradrenergic Neurons in the Locus coeruleus by Corticotropin-Releasing Factor
作者:
Carla Schulz,
Hendrik Lehnert,
期刊:
Neuroendocrinology
(Karger Available online 1996)
卷期:
Volume 63,
issue 5
页码: 454-458
ISSN:0028-3835
年代: 1996
DOI:10.1159/000127071
出版商: S. Karger AG
关键词: Corticotropin-releasing hormone;Locus coeruleus;Parietal cortex;Catecholamines;Microdialysis
数据来源: Karger
摘要:
In the present study the effects of different doses of corticotropin-releasing factor (CRF) and the CRF antagonist α-helical CRF on locus coeruleus (LC) neurons were studied in anesthetized male Wistar rats. To monitor the release of noradrenaline (NA) and its metabolite 3-methoxy-4-hydroxyphenylethylene glycol (MHPG), a microdialysis probe was implanted into the parietal cortex, a major projection area of the LC. Saline, 0.17, 0.51 nmol CRF and a combination of 5.1 nmol α-helical CRF and 0.51 nmol CRF were applied to the LC via a fused silica capillary. While both doses of CRF augmented NA in parietal cortex dialysates (0.51 nmol CRF: from 0.0206 to 0.0266 pmol/sample; 0.17 nmol CRF: from 0.0147 to 0.0170 pmol/sample), saline did not affect NA concentration. The metabolite MHPG also increased, but in a more prolonged time course. The antagonist α-helical CRF attenuated the CRF effects. The increase of extraneuronal NA concentration monitored in the cortical samples indicates an augmented depolarization rate of noradrenergic LC neurons. This clearly demonstrates the activation of these neurons by CRF, suggesting physiological interactions of CRF and noradrenergic neuro
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