Reverse-Phase High Performance Liquid Chromatographic Determination of Clidinium Bromide and Chlordiazepoxide in Tablet Formulations
作者:
I.M. Jalal,
S.I. Sa'sa',
A. Hussein,
H.S. Khalil,
期刊:
Analytical Letters
(Taylor Available online 1987)
卷期:
Volume 20,
issue 4
页码: 635-655
ISSN:0003-2719
年代: 1987
DOI:10.1080/00032718708067995
出版商: Taylor & Francis Group
关键词: Reverse Phase;HPLC;Clidinium;Chlordiazepoxide;Demoxepam;Stability;Analysis
数据来源: Taylor
摘要:
Clidinium bromide is a quaternary ammonium anticholinergic agent with peripheral effects. It is commercially available in combination with chlordiazepoxide. There is no official compendial method available for the analysis of both compounds concurrently. Conventional methods for determining both compounds involve extensive extraction and thus are time-consuming, and lack precision. The analytical scheme described in this paper provides a fast and reliable reverse-phase HPLC for chlordiazepoxide and clidinium bromide in pharmaceutical combinations. The mobile phase was 0.04 M ammonium acetate in 70% acetonitrile solution with 1% dimethylformamide (pH 6). The column was microPak MCH-10 (300.0 mm × 4.0 mm), UV detection at 254 nm and benzophenone was used as internal standard. The method was confirmed for linearity, recovery, specificity, accuracy, and applicability.
点击下载:
PDF (510KB)
返 回