Growth factor stimulation of adult articular cartilage
作者:
Keith D. Osborn,
Stephen B. Trippel,
Henry J. Mankin,
期刊:
Journal of Orthopaedic Research
(WILEY Available online 1989)
卷期:
Volume 7,
issue 1
页码: 35-42
ISSN:0736-0266
年代: 1989
DOI:10.1002/jor.1100070106
出版商: Wiley Subscription Services, Inc., A Wiley Company
关键词: Articular cartilage;Somatomedin;Growth factors;Insulin;Culture
数据来源: WILEY
摘要:
AbstractWe have examined the effect of peptide growth factors on DNA and proteoglycan synthesis by adult bovine articular cartilage in organ culture. The actions of somatomedin‐C/insulin‐like growth factor I (Sm‐C/IGF‐I), insulin, epidermal growth factor (EGF), and fibroblast growth factor (FGF) from bovine pituitary were investigated individually and in combination. FGF stimulated a 10‐fold increase in tritiated thymidine incorporation while other factors used individually did not influence mitotic activity. Used in concert, insulin with EGF and insulin with FGF acted synergistically in stimulating DNA synthesis 20‐fold and 40‐fold, respectively. All of these growth factors, acting individually, significantly enhanced radiosulfate incorporation. This stimulation was additive for Sm‐C/IGF‐I in combination with EGF or FGF, but not with insulin. These data indicate that adult bovine articular chondrocytes possess the capacity to augment both mitotic and differentiated cell functions in response to growth factors. The data further suggest that, with the exception of insulin and Sm‐C/IGF‐I, which appear to share a common mechanism of action, these factors produce their cellular effects via different receptor or
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