Mycotoxin research throughout the world focuses primarily on the problems associated with stored grains, legumes and the commodities produced from these feed stocks. While contaminated grains and legumes represent a direct threat of major importance to both human and animal consumers, they are not the only source of mycotoxins important to the agricultural sector. Grazing animals are exposed to mycotoxins produced by fungi resident in the pastures they graze, thus, in pastoral agricultural systems, a different spectrum of mycotoxicoses takes prominence. The analysis of toxins from herbage samples by conventional means (such as liquid chromatography and high‐pressure liquid chromatography) is often difficult, slow and expensive. Consequently, we have developed immunoassays which permit analysis of simple extracts and allow the rapid quantification of mycotoxins in large numbers of herbage samples. We detail enzyme‐linked immunosorbent assays using both polyclonal and monoclonal antibodies for the measurement of Fusarium toxins (zearalenone and the trichothecenes), sporidesmin, and the indole‐, diterpenoid and ergot alkaloids of endophytic fungi. Other applications using immunoanalytical techniques are also detailed.