Uptake, Intracellular Distribution, and Stability of Oligodeoxynucleotide Phosphorothioate bySchistosoma mansoni
作者:
LIANG-FENG TAO,
KENNETH A. MARX,
WARANYA WONGWIT,
ZHIWEI JIANG,
SUDHIR AGRAWAL,
ROBERT M. COLEMAN,
期刊:
Antisense Research and Development
(MAL Available online 1995)
卷期:
Volume 5,
issue 2
页码: 123-129
ISSN:1050-5261
年代: 1995
DOI:10.1089/ard.1995.5.123
数据来源: MAL
摘要:
Thein vitrouptake, cellular distribution, efflux, stability, and toxicity levels of an oligodeoxynucleotide phosphorothioate (PS-oligonucleotide) have been studied in matureSchistosoma mansoniworms. The intracellular accumulation of35S-labeled PS-oligonucleotide occurred roughly in proportion to the worm body mass over a wide concentration range, whether the worms were exposed singly or in mating pairs. Cellular uptake was dependent on the extracellular concentration. A minor fraction (13%) of the PS-oligonucleotide taken up by the worm accumulated in the surface tegumental coat. Most of the PS-oligonucleotide taken up localized in the cytosol (54%) and the nuclei-enriched (33%) fractions. In a time course study on adult worms in culture, oligonucleotide uptake was observed within the first 2 h and peaked at about 36 h. A decrease in the intracellular concentration of the PS-oligonucleotide was observed by 42 h. Analysis of the extracted oligonucleotides showed that PS-oligonucleotide was digested slowly. Efflux of the oligonucleotide was time and temperature dependent. Significant toxicity to the cultured worms did not occur until the PS-oligonucleotide concentration was over 8 mg/ml (1 mM).
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