CCK Receptor Antagonist Loxiglumide Alters Uptake of CCK in Perfused Liver and Pancreatic Acini of the Rat
作者:
K. Beckh,
R. Göke,
W. Ruff,
I. Koop,
R. Arnold,
G. Adler,
期刊:
Digestion
(Karger Available online 1991)
卷期:
Volume 48,
issue 2
页码: 98-103
ISSN:0012-2823
年代: 1991
DOI:10.1159/000200679
出版商: S. Karger AG
关键词: CCK;Hepatic extraction;Perfused rat liver;Pancreatic acini;Internalization;Loxiglumide
数据来源: Karger
摘要:
The aim of the present study was to analyze the effect of the specific cholecystokinin (CCK) receptor antagonist loxiglumide on hepatic and pancreatic processing of CCK-8 and the CCK analogue cerulein. Rat liver perfusion was performed in a non-recirculating system. CCK concentrations were measured by radioimmunoassay in perfusates from the inflow cannula (portal vein) and the outflow cannula (hepatic vein). In rat pancreatic acini, the effect of loxiglumide on internalization and surface-binding of radiolabelled CCK-8 was determined. Cerulein (20 nM, 2 nM) was extracted in a single pass through the liver by 29.7 and 25.4%, respectively. The hepatic uptake of CCK-8 (50 pM, 2 nM) was more than 90 and 89.9%, respectively. Loxiglumide drastically inhibited hepatic extraction of both peptides and reduced internalization of 125I-CCK-8 in pancreatic acini dose dependently by 39–93%. These results demonstrate that the potent CCK receptor antagonist loxiglumide significantly decreased CCK uptake by the liver and pancrea
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