Cytokeratins 8 and 18 in Smooth Muscle CellsDetection in Human Coronary Artery, Peripheral Vascular, and Vein Graft Disease andin Transplantation‐Associated Arteriosclerosis
作者:
Lothar Jahn,
Jorg Kreuzer,
Eberhard Hodenberg,
Wolfgang Kiibler,
Werner Franke,
Jens Allenberg,
Seigo Izumo,
期刊:
Arteriosclerosis and Thrombosis: A Journal of Vascular Biology
(OVID Available online 1993)
卷期:
Volume 13,
issue 11
页码: 1631-1639
ISSN:1049-8834
年代: 1993
出版商: OVID
关键词: atherosclerosis;smooth muscle cells;cytokeratins;cytoskeletal proteins
数据来源: OVID
摘要:
During development of atherosclerotic lesions, vascular smooth muscle cells (SMCs) undergo changes both phenotypically and in their cytoskeleton composition. An expression of cytokeratins 8 and 18 in SMCs in plaques of the human superficial femoral artery and of cytokeratin 8 in lesions of the aorta was recently described. Since cytokeratins are epithelial markers generally not found in normal adult vascular SMCs, we performed a detailed immunofluorescence microscopy study using a large panel of antibodies against the various cytokeratin polypeptides and other elements of the cytoskeleton. We included lesions of carotid, common and superficial femoral, iliac, and popliteal arteries; the abdominal aorta; and saphenous vein bypass grafts, as well as primary, restenotic, and transplantation-associated lesions of coronary arteries (n=33). Cytokeratins 8 and 18 were present in myointimal cells of all pathological specimens. Colocalization with smooth muscle ix-actin identified most cytokeratin-positive cells as SMCs. Only very few cells cosynthesized cytokeratin and desmin, whereas the majority of cytokeratin-positive cells were vimentin-positive. This pattern of cytoskeletal protein synthesis is similar to that found in some fetal and/or neonatal SMCs. These findings suggest that the synthesis of cytokeratins in a subset of SMCs of atherosclerotic lesions is a common phenomenon in coronary artery and peripheral vascular disease as well as graft disease and transplantation-associated arteriosclerosis and that the state of these SMCs is of a "dedifferentiated" fetal type.
点击下载:
PDF
(4145KB)
返 回