Extractionless HPLC Method for the Determination of Naproxen in Human Plasma and Urine
作者:
Th. Karidas,
A. Avgerinos,
S. Malamataris,
期刊:
Analytical Letters
(Taylor Available online 1993)
卷期:
Volume 26,
issue 11
页码: 2341-2348
ISSN:0003-2719
年代: 1993
DOI:10.1080/00032719308017474
出版商: Taylor & Francis Group
关键词: Naproxen;HPLC;Human plasma;urine
数据来源: Taylor
摘要:
A simple and rapid (extractionless) high-performance liquid chromatographic method with ultraviolet detection, at 278 nm, is described for the determination of naproxen in human plasma and urine. Niflumic acid is used as internal standard. The chromatographic system consists of a reversed-phase C18-Spherisorb column with acetonitrile/0.1 M sodium acetate (35:65 v/v, pH 6.14) as the mobile phase. The retention time is 3.0 min for naproxen and 3.8 min for niflumic acid. The total run time is 5 min and the typical assay time is 10 min. The method is sufficiently sensitive for biopharmaceutical studies, after the oral administration of a single sustained release dose.
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