Immunoenzymatic Detection of Collagen Production by Pulmonary Fibroblasts in Serum-Free Culture
作者:
O'gradyRosylnn,
KumarRakesh K.,
期刊:
Biotechnic&Histochemistry
(Taylor Available online 1994)
卷期:
Volume 69,
issue 5
页码: 283-288
ISSN:1052-0295
年代: 1994
DOI:10.3109/10520299409106303
出版商: Taylor&Francis
关键词: pulmonary fibroblasts;type I collagen;enzyme immunoassay
数据来源: Taylor
摘要:
We have developed an improved assay for the production of collagen by fibroblasts. Early passage adult mouse lung fibroblasts, established and maintained in serum-free culture, were employed as the target cells. An enzyme immunoassay was used for detection of type I collagen deposited on the substratum, permitting adaptation of the technique to cultures in 96-well microplates. Approximately two-fold enhancement of collagen deposition was induced by exposure to a concentration of 3 ng/ml of transforming growth factor-β1 or of 100 ng/ml of insulin-like growth factor-1 for 48 hr.
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