Apolipoprotein A-I of Hyperlipidemia Atherosclerosis Prone (LAP) Quail: cDNA Sequence and Tissue Expression
作者:
期刊:
Bioscience, Biotechnology, and Biochemistry
(Taylor Available online 1999)
卷期:
Volume 63,
issue 1
页码: 29-34
ISSN:0916-8451
年代: 1999
DOI:10.1271/bbb.63.29
出版商: Japan Society for Bioscience, Biotechnology, and Agrochemistry
关键词: apolipoprotein A-I;quail;mRNA;sequence;expression level
数据来源: Taylor
摘要:
Apolipoprotein A-I (apo A-I) has an important role in the transport of cholesterol. This study describes the complete nucleotide and deduced amino acid sequence for apo A-I of LAP quail. A full length apo A-I cDNA clone for hyperlipidemia atherosclerosis prone (LAP) quail was isolated from a λgt10 liver cDNA library. The DNA sequence of LAP apo A-I cDNA was similar to that of normal Japanese quail. The deduced amino acid sequence of LAP apo A-I was hence identical to that of normal Japanese quail. LAP apo A-I mRNA is about 1.4 kilobases in length and expressed in a variety of tissues including small intestine, liver, lung, breast muscle, testis, and heart. Although the tissue distribution of apo A-I was similar between strains, LAP quail expressed more apo A-I mRNA than normal Japanese quail in all tissues examined. This tendency was pronounced with the small intestine. Although the concentration of serum apo A-I did not correlate with the tissue expression of mRNA, the observation may suggest that the increased apo A-I expression in LAP strain had some relevance to the susceptibility of this strain to the experimental atherosclerosis.
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