首页   按字顺浏览 期刊浏览 卷期浏览 Human γγ‐Enolase: Two‐Site Immunoradiometric Assay with a Single Monoclonal Antibody
Human γγ‐Enolase: Two‐Site Immunoradiometric Assay with a Single Monoclonal Antibody

 

作者: B. S. Soler Federsppiel,   P. Cras,   J. Gheuens,   D. Andries,   A. Lowenthal,  

 

期刊: Journal of Neurochemistry  （WILEY Available online 1987）
卷期: Volume 48, issue 1  

页码: 22-28

 

ISSN:0022-3042

 

年代: 1987

 

DOI:10.1111/j.1471-4159.1987.tb13122.x

 

出版商: Blackwell Publishing Ltd

 

关键词: γγ‐Enolase;Enolase isozymes;Immunoassays;Monoclonal antibodies;Immunohistochemistry

 

数据来源: WILEY

 

摘要:

Abstract:A monoclonal antibody (mAb), termed BBS/NC/VI‐H14 (H14), that reacts with the human enzyme γγ‐enolase was prepared. It was directed against the γ‐subunit and did not cross‐react with the α‐ or β‐subunit. The mAb H14 can be used for quantitative determination of γγ‐enolase in a two‐site immunoradiometric assay (two‐site IRMA). It is also suitable for immunostaining formalin‐fixed tissues. The specific identification of γγ‐enolase provided by the two‐site IRMA with H14 is discussed in relation to the cellu

 

点击下载:  PDF (1408KB)



返 回