Protein synthesis in flax following inoculation with flax rust
作者:
Ben C. S. Sutton,
Michael Shaw,
期刊:
Canadian Journal of Botany
(NRC Available online 1986)
卷期:
Volume 64,
issue 1
页码: 13-18
ISSN:0008-4026
年代: 1986
DOI:10.1139/b86-004
出版商: NRC Research Press
数据来源: NRC
摘要:
Resistance to flax rustMelampsora lini(Ehrenb.) Lév. in flax carrying theNresistance gene is determined by 24 h postinoculation, at which time hypersensitivity is observed. We have examined protein synthesis in cotyledons inoculated with both virulent and avirulent races of rust byin vivolabelling with [35S]methionine. The pattern of protein synthesis was assessed by one- and two-dimensional electrophoresis 8, 13, and 18 h after inoculation. No changes in protein synthesis were observed in the first 14 h following inoculation; however, by 18 h after inoculation the susceptible combination showed a marked decrease in protein synthesis (22%;P = 0.01). This could be largely accounted for by the reduced synthesis of the ribulose 1,5-bisphosphate carboxylase large subunit, which was readily quantified on electrophoresis gels. In addition, a 30-kDa polypeptide also declined in the susceptible combination. Two-dimensional electrophoresis enabled changes to be detected in the synthesis of other minor polypeptides. None of these changes were observed in the resistant combination in which a small increase in the synthesis of the ribulose 1,5-bisphosphate carboxylase large subunit and the 30-kDa polypeptide was found. These results indicate that the outcome of the host–pathogen interaction has already been determined by 18 h after inoculation.
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