Quantification of Immunosuppression by Flow Cytometry in Stable Renal Transplant Recipients
作者:
Mario Stalder,
Tudor Bîrsan,
Bari Holm,
Mojgan Haririfar,
John Scandling,
Randall Morris,
期刊:
Therapeutic Drug Monitoring
(OVID Available online 2003)
卷期:
Volume 25,
issue 1
页码: 22-27
ISSN:0163-4356
年代: 2003
出版商: OVID
关键词: Kidney transplantation;Immunosuppression;Lymphocyte function;Flow cytometry
数据来源: OVID
摘要:
The current standard of monitoring transplant patients by drug levels is not optimal because it does not take into account the different and individual effects of immunosuppressive drugs on each patient. In this study, the authors tested immune function assays for monitoring transplant patients. Blood was collected from stable renal transplant patients treated with cyclosporin, mycophenolate mofetil, and prednisone (n = 8), and from healthy volunteers (n = 12). Lymphocyte proliferation, expression of T-cell surface activation antigens (CD25, CD71, CD11a, CD95, CD154), production of intracellular cytokines (IL-2, INF&ggr;, TNF&agr;), and lymphocyte subsets (CD4, CD8, CD16, CD20) were assessed by flow cytometry. Lymphocyte proliferation, expression of T-cell surface activation antigens, and production of intracellular cytokines were significantly decreased in transplant recipients compared with healthy control volunteers. The combined effects of several immunosuppressive drugs in renal transplant recipients can be quantitated with immune function assays in whole blood. This new method may be helpful to achieve an optimal level of immunosuppression for each patient.
点击下载:
PDF
(279KB)
返 回