AbstractCholine aecetyltransferase has been demonstrated in the duct and parenchyma of the pancreatic: gland of the cat. Potentiation of the effect of acetylcholine on the contractility of ductal segments has been observed after eserinization, indicating the presence of cholinesterase in the duct. These findings are considered to indicate that the pancreatic duct of the cat is cholinergically innervated.