Isolation of lysozyme on chitosan
作者:
Riccardo A. A. Muzzarelli,
Gaetano Barontini,
Roberto Rochetti,
期刊:
Biotechnology and Bioengineering
(WILEY Available online 1978)
卷期:
Volume 20,
issue 1
页码: 87-94
ISSN:0006-3592
年代: 1978
DOI:10.1002/bit.260200107
出版商: Wiley Subscription Services, Inc., A Wiley Company
数据来源: WILEY
摘要:
AbstractLysozyme has been immobilized on chitosan, a polyaminosaccharide, without using any intermediate reagent. The best pH conditions for operating the chitosan columns have been determined and the best eluting agent was found to be a 2% solution of propylamine. The lysozyme activity was determined after reacting lysozyme with the product of glycolchitin and Remazol Brilliant Blue R. The recovery of lysozyme from chicken egg white yields lysozyme with 55% activity.
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