Effects of Clomipramine and Verapamil on 5-HT-lnduced Intracellular Calcium Changes in Individual C6 Rat Glioma Cells
作者:
Takayuki Yamaji,
Ariyuki Kagaya,
Yasumasa Okamoto,
Teruo Hayashi,
Nobutaka Motohashi,
Shigeto Yamawaki,
期刊:
Neuropsychobiology
(Karger Available online 1996)
卷期:
Volume 33,
issue 2
页码: 55-59
ISSN:0302-282X
年代: 1996
DOI:10.1159/000119249
出版商: S. Karger AG
关键词: Ca2+antagonists;Antidepressants;Intracellular Ca2+mobilization;Affective disorder;Verapamil;Rat C6BU-1 glioma cells;Serotonin
数据来源: Karger
摘要:
The effects of antidepressants and calcium (Ca2+) antagonists on serotonin (5-HT)-induced Ca2+ increase were investigated in single C6BU-1 glioma cells with digital imaging microscopy. Application of 5-HT (100 nM – 100 µM) caused a rapid and reversible rise of intracellular Ca2+ concentration ([Ca2+]i) in a concentration-dependent manner. In the absence of extracellular Ca2+, the sustained phase of the [Ca2+]i response was strongly reduced, which was greater than the suppression of the initial peak. This suggests that the peak value is mainly due to internal Ca2+ storage sites, and the sustained phase is mainly composed of Ca2+ influx. The sustained phase was significantly attenuated by 100 nMclomipramine and verapamil. The present findings demonstrate that clomipramine and verapamil, in their therapeutic concentrations, have a common action of inhibiting Ca2+ influx, and suggest that the calcium antagonistic effect may play an important role in clinical effects of antidepressan
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