Synthesis of Polygalacturonate Trans‐eliminase and Polygalacturonase by a Strain ofEnterobacter cloacaeIsolated from Ponded Sitka Spruce
作者:
JEAN MACKEN,
A. H. PICKAVER,
期刊:
Journal of Applied Bacteriology
(WILEY Available online 1979)
卷期:
Volume 46,
issue 1
页码: 75-86
ISSN:0021-8847
年代: 1979
DOI:10.1111/j.1365-2672.1979.tb02583.x
出版商: Blackwell Publishing Ltd
数据来源: WILEY
摘要:
During the ponding of Sitka spruce in lake water there was a change from a diverse, aerobic flora (16 species) to a restricted, facultatively anaerobic flora (1 or 2 species). This change corresponded with a marked increase in the degradation of pectin and a concomitant increase in the permeability of the wood to preservatives. A strain ofEnterobacter cloacae(NCPPB 2909) isolated during ponding, synthesized an extracellular and intracellular polygalacturonase (PG) and an intracellular polygalacturonate trans‐eliminase (PGTE). Both PG and PGTE were growth–linked; extracellular PG was produced initially and then replaced by intracellular PG and PGTE. A change in the pH value of the medium did not alter the relative synthesis of enzyme. Reduced oxygen tension retarded growth but had no effect on enzyme activities. PG and PGTE ofE. cloacaewere shown to have specific ion requirements and when tap‐water was used in the preparation of a medium growth did not occur. The results are discussed in relation to an artificial system of sprinkling water, seeded with known species of bacteria, on to spruce wood in order to control the rate of pectin degradation and thus the permeability of the wood to preserva
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