A rapid spectrofluorimetrio method for the analysis of khellin and visnagin in fruits and liquid extract of Ammi visnaga has been described. Khellin is determined fluorimetrically in chloroform, where visnagin exhibits no contribution. The latter is estimated by measuring its fluorescence in ethanol, where khellin shows no interference. The method is highly selective and can be performed without prior separation of the individual furanochromones. The sensitivity range is 1 - 5 ug ml−1for each compound. The method was successfully applied to the analysis of khellin and visnagin in laboratory-made mixtures, fruits and liquid extract of Ammi visnaga.