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Antigen Retrieval Using pH 3.5 Glycine-HCI Buffer or Urea Solution for Immunohistochemical Localization of Ki-67

 

作者: RongShan,   ChaiwunBenjaporn,   YoungLillian,   ImamAshraf,   CoteRichard J.,   TaylorClive R.,  

 

期刊: Biotechnic&Histochemistry  （Taylor Available online 1994）
卷期: Volume 69, issue 4  

页码: 213-215

 

ISSN:1052-0295

 

年代: 1994

 

DOI:10.3109/10520299409106289

 

出版商: Taylor&Francis

 

数据来源: Taylor

 

摘要:

A new antibody (MIB-1) has been described, permitting the demonstration of Ki-67 proliferation antigen in paraffin sections. However, satisfactory results were obtained only after subjecting tissue sections to microwave based antigen retrieval in citrate buffer solution. Other buffer solutions produce equivalent or better results and also permit use of the original Ki-67 antibody, which hitherto has been considered ineffective for paraffin sections.

 

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