Evidence for Autocrine Basis of Transformation in NIH-3T3 Cells Transfected with met/HGF Receptor Gene
作者:
KochharKulvinder S.,
JohnsonMark E.,
VolpertOlga,
IyerAnand P.,
期刊:
Growth Factors
(Taylor Available online 1995)
卷期:
Volume 12,
issue 4
页码: 303-313
ISSN:0897-7194
年代: 1995
DOI:10.3109/08977199509028968
出版商: Taylor&Francis
关键词: HGF;met/HGF receptor;autocrine transformation;receptor-tyrosine Kinase
数据来源: Taylor
摘要:
AbstractNIH-3T3 cells transformed with met/HGF receptor gene proliferate in the absence of serum and growth factors. Immunocytochemical staining with anti-HGF antibody revealed intense HGF staining in the transfected cells. Additionally, these cells secrete bioactive HGF as evidenced by the ability of the conditioned media to stimulate met/HGF receptor phosphorylation in epithelial cells, and to promote migration of bovine adrenal capillary endothelial cells in a modified Boyden chamber assay. The migration of endothelial cells could be specifically inhibited by anti-HGF antibody but not by an irrelevant antibody. Suramin, a drug known to disrupt ligand-receptor interactions, inhibits the serum and growth-factor free proliferation, and the endogenous phosphorylation of met/HGF receptor in the transformed cells. Taken together, our data suggests an autocrine mode of transformation in NIH-3T3 cells transfected with met/HGF receptor gene.
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