In Situ Embedding of Cell Monolayers Cultured on Plastic Surfaces for Electron Microscopy
作者:
MurrayA. Beatrice,
SchulzeHelga,
BlauwElisabeth,
期刊:
Biotechnic&Histochemistry
(Taylor Available online 1991)
卷期:
Volume 66,
issue 5
页码: 269-272
ISSN:1052-0295
年代: 1991
DOI:10.3109/10520299109109985
出版商: Taylor&Francis
关键词: in situ embedding;cell monolayers;electron microscopy
数据来源: Taylor
摘要:
Various procedures suitable for routine in situ embedding of cell monolayers were tested including: (1) the use of different Epon substitutes, (2) the use of different types of plas-ticware obtained from different sources, and (3) different methods of preparing capsules for sectioning. Different resins reacted differently with different plastics and type of preparation. Merck Epon substitute bound to most of the plastics tested. Ladd Epon substitute released cleanly from all plastics tested when a suitable method of preparation was used. The results show that for routine embedding of cell monolayers it is necessary to select an appropriate Epon substitute and method of preparation of capsules for the type of plasticware used. A routine method is described, with various alternative steps which can be applied when particular difficulties are encountered.
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