Demonstration of Surface Antigens on Bronchoalveolar Lavage Cells Using the Immunoalkaline Phosphatase Method
作者:
A. Xaubet,
C. Agustí,
C. Picado,
A. Urbano-lspizuá,
M. Carrión,
A. Agustí-Vidal,
期刊:
Respiration
(Karger Available online 1989)
卷期:
Volume 56,
issue 1-2
页码: 43-47
ISSN:0025-7931
年代: 1989
DOI:10.1159/000195776
出版商: S. Karger AG
关键词: Bronchoalveolar lavage;Immunoalkaline phosphatase;Lung T-cell subsets
数据来源: Karger
摘要:
The immunoalkaline phosphatase procedure is described as a method for labelling bronchoalveolar lavage cellular specimens with monoclonal antibodies. This method has several advantages over conventional immunofluroescent techniques: it can be performed on cytocentrifuge preparations stored for long periods before staining; cell morphology can be observed in detail in positive and negative cells; the staining is permanent and stable, and, the reaction can be evaluated with a light microscope. Normal values for lymphocyte subpopulations in smokers and nonsmokers are also reported.
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