In Vivo Cytokine Gene Expression in Various T Cell Subsets of the Autoimmune MRL/Mp-lpr/lpr Mouse
作者:
MoriK.,
KobayashiS.,
InobeM.,
JiaW. Y.,
TamakoshM.,
MiyazakiT.,
UedeT.,
期刊:
Autoimmunity
(Taylor Available online 1994)
卷期:
Volume 17,
issue 1
页码: 49-57
ISSN:0891-6934
年代: 1994
DOI:10.3109/08916939409014658
出版商: Taylor&Francis
关键词: lpr mice;double negative T cells;cytokine;Eta-1
数据来源: Taylor
摘要:
We have used the reverse transcriptase polymerase chain reaction (RT-PCR) technique to assess the expression of cytokine genes in various T cell subsets of autoimmune-prone mice. Our study confirmed the previously described features in lpr mice that IFN-γ, TNF-α, and TNF-βwere transcribed by various T cell subsets. We in this study demonstrated that double negative (DN) T cells, the major cell population in lpr mice, failed to express interleukin-3 (IL-3), IL-4, IL-5, and IL-6 genes that influence B cell growth and activation. In contrast, DN T cells expressed Eta-1 gene that is shown to augment polyclonal activation of B cells and immunoglobulin production. Thus, it is conceivable that T cell-derived B cell-stimulatory activities in MRL/lpr mice can be attributed to Eta-1, rather than IL-3, IL-4, IL-5 or IL-6. We also demonstrated that CD4+T cells infiltrating into kidney tissues of MRL/Ipr mice expressed various cytokine genes such as IL-1, IL-5, IL-6, IFN-γ, TNF-α, TNF-βand TGF-p.
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