Correction of fluorescence detected circular dichroic photoselection artifacts using light collecting ellipsoidal mirrors
作者:
S. E. Bicknese,
M. F. Maestre,
期刊:
Review of Scientific Instruments
(AIP Available online 1987)
卷期:
Volume 58,
issue 11
页码: 2060-2063
ISSN:0034-6748
年代: 1987
DOI:10.1063/1.1139463
出版商: AIP
数据来源: AIP
摘要:
Ellipsoidal mirrors are used to collect the emitted light from fluorophores in fluorescence detected circular dichroic (FDCD) measurements and to deliver this light to a photomultiplier tube (PMT) at 90° to the excitation beam. These mirrors collect light over a symmetrical 71% of the emission sphere centered on the sample cell, and thereby eliminate most of the nonuniformity in detected emission resulting from the anisotropy of photoselection. Furthermore, even with reflectance and path length losses, the amount of fluorescent emission reaching the PMT is increased by a factor of approximately 18. Elimination of photoselection artifacts in UV FDCD spectra is demonstrated by comparing spectra of L‐tryptophan in glycerol with its spectra in water. Validity of the values measured is verified through calibration with a d10 camphorsulfonic acid (d10 CSA) standard.
点击下载:
PDF
(335KB)
返 回