Early Treatment with a Novel Inhibitor of Lipid Peroxidation (LY341122) Improves Histopathological Outcome after Moderate Fluid Percussion Brain Injury in Rats
作者:
Kojiro Wada,
Ofelia Alonso,
Raul Busto,
Jill Panetta,
James Clemens,
Myron Ginsberg,
W. Dietrich,
期刊:
Neurosurgery
(OVID Available online 1999)
卷期:
Volume 45,
issue 3
页码: 601-601
ISSN:0148-396X
年代: 1999
出版商: OVID
关键词: Fluid percussion brain injury;Free radical scavenger;Lipid peroxidation inhibitor;LY341122;Rat
数据来源: OVID
摘要:
OBJECTIVEReactive oxygen species are thought to participate in the pathobiology of traumatic brain injury (TBI). This study determined whether treatment with LY341122, a potent inhibitor of lipid peroxidation and an antioxidant, would provide neuroprotection in a rat model of TBI.METHODSTo investigate the efficacy of LY341122 in this parasagittal fluid percussion model (1.8–2.1 atm), the rats received oral administration of LY341122 (100 mg/kg) or vehicle 2 hours before and 4 hours after TBI (each group, n = 7). To investigate the therapeutic window for treatment, rats were treated with LY341122 or vehicle for 20 hours by femoral vein infusion starting at 5 minutes, 30 minutes, or 3 hours after TBI (each group, n = 5). Three days after injury, analysis of contusion volumes and the frequency of damaged cortical neurons was conducted.RESULTSOral administration of LY341122 before and after TBI led to a significant reduction in overall contusion volume (3.28 mm3± 0.75 mm3[mean ± standard error of the mean] versus 1.32 mm3± 0.33 mm3;P< 0.05) and also reduced the frequency of damaged cortical neurons (1191.7 ± 267.1 versus 474.6 ± 80.2;P< 0.05). In the second experiment, rats treated with LY341122 at 5 minutes or 30 minutes after TBI also demonstrated a significant reduction (P< 0.05) in contusion volume (1.92 mm3± 0.64 mm3or 1.59 mm3± 0.50 mm3, respectively) compared with vehicle-treated rats (4.32 mm3± 1.15 mm3). A significant reduction in total cortical necrotic neuron counts was also demonstrated in the 5-minute group (2243.8 ± 265.3 versus 1457.8 ± 265.3;P< 0.05). In contrast, histopathological outcome was not significantly improved when treatment was delayed until 3 hours after TBI.CONCLUSIONThese data reinforce the hypothesis that lipid peroxidation and reactive oxygen species participate in the acute pathogenesis of TBI. Treatment delayed until 3 hours after TBI did not provide significant histopathological protection.
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