Ozone-induced metabolic changes in Scots pine: mRNA isolation and analysis of in vitro translated proteins
作者:
E. Grobkopf,
A. Wegener-Strake,
H. Sandermann Jr.,
D. Ernst,
期刊:
Canadian Journal of Forest Research
(NRC Available online 1994)
卷期:
Volume 24,
issue 10
页码: 2030-2033
ISSN:0045-5067
年代: 1994
DOI:10.1139/x94-260
出版商: NRC Research Press
数据来源: NRC
摘要:
A rapid isolation procedure for intact poly(A)+RNA from Scots pine (PinussylvestrisL.) is described. Translation products of poly(A)+RNA isolated from 6-week-old Scots pine seedlings treated with ozone (0.3 μL/L) for 8 h were analyzed using two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). Both qualitative and quantitative differences in the translation products were observed after ozone fumigation. Expression of specific mRNAs, coding for stress-induced polypeptides between 12 kD and 50 kD, was demonstrated after 8 h of ozone treatment.
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