Analysis of carbohydrates by HPLC with post‐column derivatization
作者:
Michael Krämer,
Heinz Engelhardt,
期刊:
Journal of High Resolution Chromatography
(WILEY Available online 1992)
卷期:
Volume 15,
issue 1
页码: 24-29
ISSN:0935-6304
年代: 1992
DOI:10.1002/jhrc.1240150107
出版商: Wiley Subscription Services, Inc., A Wiley Company
关键词: Carbohydrate analysis;Oligosaccharides;Post‐column derivatization
数据来源: WILEY
摘要:
AbstractPost‐column derivatization of oligosaccharides after HPLC separation with a variety of systems has been achieved either by reaction with thymol in concentrated sulfuric acid or after hydrolysis of saccharides with hydrochloric acid and derivatization of the resulting monosaccharides withp‐aminobenzoic acid hydrazide. With both reactions the detection of reducing and non‐reducing sugars is possible even at trace levels. The latter reaction, despite the two reaction steps required, is much more convenient and practical, whereas with the first reaction it is also possible to determine alkylpolygluco
点击下载:
PDF
(495KB)
返 回