Pulsed field gel electrophoresis runs were performed in small Contour Clamped Homogeneous Electric Field (miniCHEF) and Transverse Alternating Electric Field (miniTAFE) chambers. Minichambers use minigels and they could be suitable for analytical and preparative purposes. In minichambers higher electric fields and equal heat evolution were achieved with the same voltage applied to conventional chambers. Nine to tenSaccharomyces cerevisiaechromosomes were resolved in minichambers applying 13–14 V/cm and 50 s of pulse time during 3.2 - 5 h. It was found a linear relationship between the migration per pulse and the electric field (at constant pulse time). An equation relating the pulse time and the electric fields was attained. It was used to obtain isopatterns at several electric fields despite the chamber dimensions. Miniapparati use simple power supplies and cooling systems and save samples, reagents, laboratory bench space and time.