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Optimization of Total Thyroxine Radioimmunoassay in Unextracted Serum by Dextran Charcoal

 

作者: Y. M. Megahed,   G. A. Ziada,   M. M. Mahfouz,   M. Abdel-Razak,   S. E. El-Hadad,  

 

期刊: Isotopenpraxis Isotopes in Environmental and Health Studies  （Taylor Available online 1979）
卷期: Volume 15, issue 9  

页码: 282-287

 

ISSN:0021-1915

 

年代: 1979

 

DOI:10.1080/10256017908544353

 

出版商: Taylor & Francis Group

 

关键词: blood serum;charcoal;comparative evaluations;dextran;diagnosis;hyperthyroidism;hypothyroidism;iodine 125;optimization;patients;radioimmunoassay;radiopharmaceuticals;sensitivity;thyroxine

 

数据来源: Taylor

 

摘要:

Specific method for accurate radioimmunoassay of free and total thyroxine in un-extracted serum has been developed. The method is used for estimation of either free thyroxine (T4) or total T4by inhibition of the binding sites of T4to protein using TCA/NaOH mixture. The antibodies used in this study were obtained from Mallinkrodt. The separation technique used in our modification is that of dextran charcoal, this is to absorb the free fractions on charcoal.

 

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