首页   按字顺浏览 期刊浏览 卷期浏览 Neisseria-gonorrhoeae-Züchtung: Entwicklung einer vereinfachten Methode
Neisseria-gonorrhoeae-Züchtung: Entwicklung einer vereinfachten Methode

 

作者: Z.S. Itani,  

 

期刊: Dermatology  (Karger Available online 1977)
卷期: Volume 154, issue 5  

页码: 273-276

 

ISSN:1018-8665

 

年代: 1977

 

DOI:10.1159/000251081

 

出版商: S. Karger AG

 

关键词: Neisseria gonorrhoeae;Easy method;Co2development;Venereal disease;Stool tube

 

数据来源: Karger

 

摘要:

An easy and cheap method for culturing Neisseria is developed. The medium for gonococci is prepared as proposed by the producer (BBL, Oxoid, Hoechst). About 8 ml of the medium are poured in sterile air-tight stool tubes of 25 ml volume. Materials to be examined for gonococci are taken from the cervix, ureter or anus and are inoculated on the medium. A small piece (20–30 mg) of the GasPak tablet (BBL) is then deposited in the tube and closed immediately. The GasPak tablet consists of sodium bicarbonate and citric acid which, if they come in contact with humidity, produce Co2 gas. The inoculated tube is then put in an incubator at 37°C for 14–24 h. This method gives a good microbiological result. With the aid of the oxidase reaction the colonies take a brown-black color. For further differentiation of the species the sugar fermentation method is neces

 

点击下载:  PDF (694KB)



返 回