Fluorescence immunohistochemical detection of Armillaria and Heterobasidion in Norway spruce
作者:
U. Zollfrank,
C. Sautter,
B. Hock,
期刊:
European Journal of Forest Pathology
(WILEY Available online 1987)
卷期:
Volume 17,
issue 4‐5
页码: 230-237
ISSN:0300-1237
年代: 1987
DOI:10.1111/j.1439-0329.1987.tb01021.x
出版商: Blackwell Publishing Ltd
数据来源: WILEY
摘要:
AbstractArmillaria melleass. lato andHeterobasidion annosumwere identified in infected Norway spruce using an indirect immunohistochemical test. Unspecific binding sites were masked by incubating the specimen with preimmune serum followed by protein A. Subsequent labeling with monospecific rabbit antibodies and fluorochrome‐conjugated protein A yielded a strong and specific fluorescent signa
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