GC Behavior of 3-Ketosteroid Methoximes. Application to GC Studies of Adrenocortical Steroid Hormone MO-TMS Derivatives
作者:
J.-P. Thenot,
E.C. Horning,
期刊:
Analytical Letters
(Taylor Available online 1972)
卷期:
Volume 5,
issue 11
页码: 801-814
ISSN:0003-2719
年代: 1972
DOI:10.1080/00032717208069562
出版商: Taylor & Francis Group
关键词: 3-ketosteroids;corticouteroids;methoxime;methoxime-trimethylsilyl derivatives;Beckmann fission;gas chromatography;mass spectrometry
数据来源: Taylor
摘要:
3-Ketosteroid methoximes (MO derivatives) undergo a degradation when injected into columns that contain acidic materials or supports with acidic sites. Degradation products can usually not be isolated; they result from a Beckmann fission reaction. The extent of sample loss is dependent on the amount of acidic material (pyridine hydrochlo-ride), and decreases with time as the column is “healed” by sweeping or silylation treatment. Lanost-8-ene-3-one MO derivative, where the methoxime group is adjacent to a tertiary carbon atom, gives an unsaturated nitrile by Beckmann fission. An appropriate analytical technique was developed to prevent sample loss. About 1–3 cm of 10% coated support at the head of the column will prevent permanent damage to the column; the solution of the analytical sample should also contain an excess of silylation reagent. When this is done, no sample loss is observed in GC studies of adrenocortical steroid hormones as MO-TMS derivatives.
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