A New Method for the HPLC Analysis of Pt(II) in Urine
作者:
RichardF. Borch,
JeromeH. Markovitz,
MichaelE. Pleasants,
期刊:
Analytical Letters
(Taylor Available online 1979)
卷期:
Volume 12,
issue 8
页码: 917-926
ISSN:0003-2719
年代: 1979
DOI:10.1080/00032717908059771
出版商: Taylor & Francis Group
关键词: Cis-Dichlorodiammineplatinum;HPLC;Dithiocar-bamate;Clinical Assay
数据来源: Taylor
摘要:
An analytical method has been developed to measure Pt(II) in urine via derivatization and UV or HPLC analysis. A measured quantity of urine is heated briefly with diethyl ammonium diethyl-dithiocarbamate, and the resulting Pt(Et2NCS2)2is extracted into a measured volume of chloroform. Concentrations of Pt(II) are determined by UV absorption at 346 nm or by reverse phase HPLC analysis. The detection limit for Pt(II) as its dithiocarbamate is ∼ 1 ng by HPLC; the concentration limit for HPLC analysis by direct extraction was ∼ 25 ng/ml. Chromatographic response was linearly related to Pt(II) concentration over the range 100-4, 000 ng/ml; dilution of more concentrated samples has extended this range to at least 30, 000 ng/ml. This method has been applied to the analysis of Pt(II) in the urine of patients who have received cis-dichlorodiamniineplatinum(II) (CDDP) chemotherapy.
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