The identification and localization of a catecholamine in the motor neurons of the lobster cardiac ganglion
作者:
Karen A. Ocorr,
Allan Berlind,
期刊:
Journal of Neurobiology
(WILEY Available online 1983)
卷期:
Volume 14,
issue 1
页码: 51-59
ISSN:0022-3034
年代: 1983
DOI:10.1002/neu.480140107
出版商: Wiley Subscription Services, Inc., A Wiley Company
数据来源: WILEY
摘要:
AbstractThe cardiac ganglion fromHomarus americanuswas investigated for the purpose of providing biochemical and histochemical information as to the identity of the neurotransmitter(s) utilized by this system. Three techniques were employed in this study: (1) the glyoxylic acid histofluorescence staining technique (GA), which showed fluorescence characteristic of catecholamines localized in the five motor neurons; (2) high‐voltage electrophoresis (HVE) in one dimension followed by ascending chromatography in the second dimension, which indicated incorporation of label from tritiated tyrosine into norepinephrine (NE) and small amounts of dopamine (DA); (3) high‐pressure liquid chromatography with electrochemical detection (HPLC/EC), which indicated the presence of endogenous norepinephr
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