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Why Do Some Stains Fade? Part I. History, Fastness Ratings, Light Absorption, and Fluor...
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Why Do Some Stains Fade? Part I. History, Fastness Ratings, Light Absorption, and Fluorochromes
作者:
PuchtlerHolde,
McGowanAmy L.,
MeloanSusan N.,
期刊:
Journal of Histotechnology
(Taylor Available online 1989)
卷期:
Volume 12,
issue 1
页码: 57-61
ISSN:0147-8885
年代: 1989
DOI:10.1179/his.1989.12.1.57
出版商: Taylor&Francis
数据来源: Taylor
摘要:
AbstractSome dyes used in histotechnology fade within months; others remain unaltered for many years. Problems of fading are well known since antiquity and have been thoroughly investigated. Yet textbooks of histotechnology do not provide information concerning the sensitivity of dyes to light, acids, alkalis, and other reagents, though quantitative data are easily accessible in technical and chemical publications. Investigations in this laboratory demonstrated the usefulness of these data for the design of new reactions and modifications of traditional technics to prevent or minimize fading. It seemed, therefore, of interest to review pertinent literature on fastness properties of dyes. This report will deal mainly with standards of the fastness of dyes to light, acids and alkalis, effects of different light sources, dye concentration, and fluorochromes. (The J Histotechno1 12:57, 1989.)
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