In the course of our biosynthetic studies of constituents of hairy root cultures of Tagetes patula, we isolated four bithiophenes,[5-(3-buten-1-yny1)]-2,2′-bithiophenes(BBT),5-(4-acetoxy-1-butyny1)-2,2′-bithiophenes (BBTOAc),5-(4-hydroxy-1-butyny1)-2,2′bithiophenes (BBTOH) and 5-(3,4-diacetoxy-1-butyny1)-2,2′-bithiophenes [BBT(OAc)2]. In addition, stigmasterol, α-farnesene, three known benzofurans, of which only one was previouly isolated from T.patula, as well as a new benzofuran were obtained. The structures of the componds were determined by spectral analysis and comparison with published data. The molecular structure of the benzofuran isoeuparin was established by single crystal x-ray diffraction.