SUMMARYNephrochytriam amazonensisoccurs as a saprophyte in decaying vegetable debris in moist soil samples collected from the Rio Candeias in Amazonas, Brazil. It is strikingly similar in structure to species ofDiplophlyctis, but forms an operculum in the exit tube after the tip has deliquesced and become filled with a plug of hyaline material. Because of the presence of an operculum it is included in the genusNephrochytrium.