Vertical transmission of HIV‐1lack of detectable virus in peripheral blood cells of infected children at birth
作者:
Anita Rossi,
Lucia Ometto,
Fabrizio Mammano,
Carlo Zanotto,
Carlo Giaquinto,
Luigi Chieco-Bianchi,
期刊:
AIDS
(OVID Available online 1992)
卷期:
Volume 6,
issue 10
页码: 1117-1120
ISSN:0269-9370
年代: 1992
出版商: OVID
关键词: Vertical HIV-1 transmission;early diagnosis of HIV-1;polymerase chain reaction
数据来源: OVID
摘要:
ObjectiveTo evaluate the time-course of HIV-1 detection in peripheral blood mononuclear cells (PBMC) from newborns at risk of vertically acquired infection.Design and methodForty-six infants born to HIV-1-infected mothers were enrolled at birth and examined virologically and clinically in the perinatal period and every 30 days for the first 3 months of life. Follow-up was conducted at intervals of 2–3 months. HIV-1 detection in PBMC was performed using virus culture and polymerase chain reaction (PCR) assay.ResultsOnly one out of 24 newborns tested within 48 h of delivery and two out of 22 infants tested between 3 and 15 days of age were found to be HIV-1-positive by both PCR and virus culture. Further testing performed between 30 and 60 days of life identified an additional eight HIV-1-positive children. Subsequent viral, immunological and clinical follow-up confirmed PCR and virus culture results obtained in 30–60-day-old children.ConclusionsInfected infants had detectable levels of HIV-1 in their PBMC at 1 month of age. The negative PCR and virus culture findings in PBMC of newborns indicate strongly that HIV-1 cannot be diagnosed at birth in the majority of cases, and suggests that viral transmission could occur during late pregnancy and/or delivery.
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