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Proximate Determination of Triterpene Glycosides inGlycyrrhizae Radixby Ion Exchange Chromatography and Turbidimetry

 

作者: Sheng‐Chih Chen,   Han‐Teh Li,  

 

期刊: Journal of the Chinese Chemical Society  (WILEY Available online 1982)
卷期: Volume 29, issue 1  

页码: 47-53

 

ISSN:0009-4536

 

年代: 1982

 

DOI:10.1002/jccs.198200007

 

出版商: WILEY‐VCH Verlag

 

关键词: glycyrrhiza;triterpene glycoside;glycyrrhizin;glycyrrhetinic acid;SP‐Sephadex;QAE‐Sephadex;turbidimetry;high performance chromatography

 

数据来源: WILEY

 

摘要:

AbstractA simple method for the proximate determination of triterpene glycosides in glycyrrhiza was developed. Glycyrrhiza extract was directly chromatographed on SP‐Sephadex C‐25 (H+) and QAE‐Sephadex A‐25 (Cl−) with distilled water and 0.2Msodium chloride (pH 13.50) as the eluents, respectively. The eluate was then hydrolyzed with perchloric acid and determined turbidimetrically at 530nm.With this method, glycyrrhiza samples collected in Taiwan and Singapore were found containing 17.21±0.45% and 7.49±0.35% of triterpene glycosides, calculated as glycyrrhizin, r

 

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