HIV‐1 viral DNA load in peripheral blood mononuclear cells from seroconverters and long‐term infected individuals
作者:
Suzanne Jurriaans,
John Dekker,
Anthony de Ronde,
期刊:
AIDS
(OVID Available online 1992)
卷期:
Volume 6,
issue 7
页码: 635-642
ISSN:0269-9370
年代: 1992
出版商: OVID
关键词: HIV;polymerase chain reaction;viral DNA load
数据来源: OVID
摘要:
ObjectiveTo determine viral DNA load in peripheral blood mononuclear cells (PBMC) from HIV-1-infected individuals.DesignHIV-1 copy numbers were determined using a quantitative polymerase chain reaction (PCR), the PCR-aided template titration assay (PATTY). PATTY utilizes an internal plasmid control DNA, which is amplified within the same tube and using the same primers as the PBMC target DNA. HIV-1 copy numbers were confirmed by limiting-dilution PCR analysis.ResultsPBMC viral load of 19 long-term (>4 years) HIV-1-infected individuals ranged from 0.8 to 100 copies per 103PBMC. Significantly higher copy numbers were found among p24-antigen-positive than among p24-antigen-negative individuals. In addition, the PBMC viral load of two HIV-1-infected individuals was monitored during the first 3 months after acute infection. For both patients, the HIV-1 copy numbers were shown to peak at the time of HIV-1-antibody seroconversion and decline subsequently (range, 0.6–10 copies per 103PBMC).ConclusionsATTY is a useful method for assessing the HIV-1 copy numbers in PBMC DNA. Viral DNA load peaks shortly after infection and reaches an individual specific level that is probably stable within a few months of infection. Viral DNA load in PBMC varies widely among long-term HIV-1-infected individuals.
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