Stereological-Ultrastructural Study of Pancreatic B Cells in Metabolic Alkalosis
作者:
María C. Semino,
Oscar R. Rebolledo,
César L.A. Gómez Dumm,
Juan J. Gagliardino,
期刊:
Cells Tissues Organs
(Karger Available online 1983)
卷期:
Volume 117,
issue 4
页码: 355-361
ISSN:1422-6405
年代: 1983
DOI:10.1159/000145808
出版商: S. Karger AG
关键词: Ultrastructure;Insulin secretion;B cell;Intracellular hormone degradation;Metabolic alkalosis
数据来源: Karger
摘要:
The secretion of insulin in response to glucose and the changes in the B cell at the ultrastructural level were studied in rat pancreas perfused at pH 7.4 and 7.8 with different concentrations of glucose. Raising the extracellular pH from 7.4 to 7.8 significantly inhibits glucose-induced insulin secretion. Coincidently, morphometric studies showed significant evidences of low secretory activity in B cells from pancreas submitted to high glucose stimulation under alkalosis, namely lower number of emiocytotic figures and microtubules as well as a decrease in the volume density of the granular endoplasmic reticulum and the Golgi complex. On the other hand, a significant increment in the number of images of granulolysis was also demonstrated. These secretory and ultrastructural results confirm the inhibitory effect of pH 7.8 upon B cell secretory activity induced by glucose. Moreover, they lend further support to the role of intracellular hormone degradation as a regulator of B cell insulin content.
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