Helicobacter pyloriis probably the most common chronic bacterial infection of humankind, and is usually acquired first in childhood. Microbiologic culture ofH. pyloriis the “gold standard” for diagnosis in a patient with suspected infection. Although not currently recommended for routine use, culture allows testing for susceptibility to antimicrobials, especially in populations with a high prevalence of drug resistance. Gastric biopsies are the specimens most commonly used to cultureH. pylori, but stool, vomitus, saliva, and dental plaque offer opportunities. This review examines the current methods used to cultureH. pylorifrom biologic specimens and suggests useful hints to enhance its recovery rate.