Cyclic GMP evoked calcium transients in olfactory receptor cell growth cones
作者:
K Kafitz,
T Leinders-Zufall,
F Zufall,
C Greer,
期刊:
NeuroReport
(OVID Available online 2000)
卷期:
Volume 11,
issue 4
页码: 677-681
ISSN:0959-4965
年代: 2000
出版商: OVID
关键词: Axon guidance;Cyclic nucleotides;Growth cones;Olfactory pathway
数据来源: OVID
摘要:
Nitric oxide-induced calcium transients in growth cones are believed to be mediated by cyclic nucleotides. Because nitric oxide is thought to influence the development of olfactory receptor cells (ORCs), we have begun to explore the effect of cyclic nucleotides on ORC growth cones. Cultured ORCs were loaded with fluo-3 AM and confocal imaging was employed to monitor calcium transients following cyclic nucleotide-gated channel activation. Application of 8-bromo-cGMP at the growth cone caused transient increases influorescence which were restricted to the growth cone and lasted tens of seconds. The signal was abolished by LY83583, an inhibitor of cyclic nucleotide-gated channels. 8-Bromo-cGMP also inhibited further extension of growth cones. The data indicate that ORC growth cones exhibit cGMP-dependent calcium transients that are consistent with those generated by cyclic nucleotide-gated channels.
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