The Role of Hemopoietic Growth Factors in Self-Renewal and Differentiation of IL-3-Dependent Multipotential Stem Cells
作者:
HeyworthC. M.,
DexterT. M.,
KanO.,
WhettonA. D.,
期刊:
Growth Factors
(Taylor Available online 1990)
卷期:
Volume 2,
issue 2
页码: 197-211
ISSN:0897-7194
年代: 1990
DOI:10.3109/08977199009071506
出版商: Taylor&Francis
关键词: stem cells;differentiation;hemopoietic growth factors;interleukin-3
数据来源: Taylor
摘要:
AbstractA multipotent hemopoietic cell line has been employed to assess the influence of the hemopoietic growth factors, IL-3, GM-CSF, G-CSF, and CSF-1 on the processes of self-renewal, the generation of lineage restricted progenitor cells and the production of mature neutrophils and macrophages. At a high concentration of IL-3, the cells undergo self-renewal and demonstrate little or no ability to undergo differentiation in the presence of the other growth factors. In the absence of IL-3, the cells show minimal (GM-CSF) or no (G-CSF or CSF-1) ability to respond to these other growth factors. When combined with a low concentration of IL-3, the ability of the cells to respond to GM-CSF, G-CSF, and CSF-1 is enhanced and a selective preference for the neutrophil or macrophage lineage is seen depending on the combination used, i.e., the presence of CSF-1 preferentially promotes macrophage development and G-CSF preferentially promotes neutrophil development. Conditions optimal for neutrophil development were seen using a combination of low IL-3 concentrations plus GM-CSF plus G-CSF. In such conditions, the cells undergo extensive proliferation and progressively lose their clonogenic potential (i.e., differentiation self-renewal) and acquire the biochemical markers characteristic of fully mature phagocytes.
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